Modification:Article Title: KLK8/HGF/Met signaling pathway mediates diabetes-associated hippocampal neuroinflammation in male mice
Article Snippet: .. BV2 mouse microglial cell lines were purchased from Servicebio (Wuhan, China) and cultivated in Dulbecco's modified Eagle's medium (DMEM, Gibco, New York, USA) containing normal glucose (NG, 5.5 mM D-glucose),10% fetal bovine serum (FBS, Gibco, New York, USA), and 1% penicillin/streptomycin (Gibco, New York, USA) at 37 °C in a humidified atmosphere consisting of 95% O 2 and 5% CO 2 . .. KLK8 adenovirus (Ad-KLK8) was generated by employing the AdEasyTM adenoviral vector system (Stratagene, La Jolla, CA, USA) as previously described .
Article Title: KLK8/HGF/Met signaling pathway mediates diabetes-associated hippocampal neuroinflammation in male mice.
Article Snippet: .. Cell culture and infection of adenovirus or lentivirus BV2 mouse microglial cell lines were purchased from Servicebio (Wuhan, China) and cultivated in Dulbecco's modified Eagle's medium (DMEM, Gibco, New York, USA) containing normal glucose (NG, 5.5 mM D-glucose),10% fetal bovine serum (FBS, Gibco, New York, USA), and 1% penicillin/ streptomycin (Gibco, New York, USA) at 37 °C in a humidified atmosphere consisting of 95% O2 and 5% CO2. .. KLK8 adenovirus (Ad-KLK8) was generated by employing the AdEasyTM adenoviral vector system (Stratagene, La Jolla, CA, USA) as previously described [11].
Cell Culture:Article Title: KLK8/HGF/Met signaling pathway mediates diabetes-associated hippocampal neuroinflammation in male mice.
Article Snippet: .. Cell culture and infection of adenovirus or lentivirus BV2 mouse microglial cell lines were purchased from Servicebio (Wuhan, China) and cultivated in Dulbecco's modified Eagle's medium (DMEM, Gibco, New York, USA) containing normal glucose (NG, 5.5 mM D-glucose),10% fetal bovine serum (FBS, Gibco, New York, USA), and 1% penicillin/ streptomycin (Gibco, New York, USA) at 37 °C in a humidified atmosphere consisting of 95% O2 and 5% CO2. .. KLK8 adenovirus (Ad-KLK8) was generated by employing the AdEasyTM adenoviral vector system (Stratagene, La Jolla, CA, USA) as previously described [11].
Infection:Article Title: KLK8/HGF/Met signaling pathway mediates diabetes-associated hippocampal neuroinflammation in male mice.
Article Snippet: .. Cell culture and infection of adenovirus or lentivirus BV2 mouse microglial cell lines were purchased from Servicebio (Wuhan, China) and cultivated in Dulbecco's modified Eagle's medium (DMEM, Gibco, New York, USA) containing normal glucose (NG, 5.5 mM D-glucose),10% fetal bovine serum (FBS, Gibco, New York, USA), and 1% penicillin/ streptomycin (Gibco, New York, USA) at 37 °C in a humidified atmosphere consisting of 95% O2 and 5% CO2. .. KLK8 adenovirus (Ad-KLK8) was generated by employing the AdEasyTM adenoviral vector system (Stratagene, La Jolla, CA, USA) as previously described [11].
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